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trypsin edta solution  (Beyotime)


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    Structured Review

    Beyotime trypsin edta solution
    Trypsin Edta Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypsin+edta+solution/Trypsin-EDTA+Solution/pm42883269-195-5-36
    Average 97 stars, based on 1195 article reviews
    trypsin edta solution - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    BIA-KA:

    Article Title: Antibacterial Performance of Defect-Enriched PtPdCuFe High-Entropy Alloy Nanozymes.
    Article Snippet: Tungsten hexacarbonyl (W(CO)6), oleylamine (OAm), glucose, polyvinylpyrrolidone (PVP), 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 2,2,6,6-tetramethylpiperidine (TEMP), sodium hypochlorite (NaClO), L-methionine, riboflavin, EDTA, and nitroblue tetrazolium chloride (NBT) were purchased from Macklin (Shanghai, China). .. DMEM, fetal bovine serum (FBS), trypsin–EDTA solution, Calcein-AM, propidium iodide (PI), DCFH-DA ROS probe, BCA protein assay kit, hydrogen peroxide assay kit, TMB assay kit, MB assay kit, and DMAO bacterial fluorescent dye were purchased from Beyotime (Shanghai, China). .. LB broth and LB agar were obtained from Solarbio (Beijing, China).

    H2O2 Assay:

    Article Title: Antibacterial Performance of Defect-Enriched PtPdCuFe High-Entropy Alloy Nanozymes.
    Article Snippet: Tungsten hexacarbonyl (W(CO)6), oleylamine (OAm), glucose, polyvinylpyrrolidone (PVP), 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 2,2,6,6-tetramethylpiperidine (TEMP), sodium hypochlorite (NaClO), L-methionine, riboflavin, EDTA, and nitroblue tetrazolium chloride (NBT) were purchased from Macklin (Shanghai, China). .. DMEM, fetal bovine serum (FBS), trypsin–EDTA solution, Calcein-AM, propidium iodide (PI), DCFH-DA ROS probe, BCA protein assay kit, hydrogen peroxide assay kit, TMB assay kit, MB assay kit, and DMAO bacterial fluorescent dye were purchased from Beyotime (Shanghai, China). .. LB broth and LB agar were obtained from Solarbio (Beijing, China).

    Isolation:

    Article Title: hUCMSC mitochondrial EVs confer neuroprotection after ischemia by Tom1l2-mediated mitochondrial fusion and Crls1-cardiolipin axis reprogramming.
    Article Snippet: .. Mitochondria were isolated from treated PC12 cells and fresh rat brain tissue using the Cell Mitochondrial Isolation Kit (Beyotime, Cat. No. C3601) and Tissue Mitochondrial Isolation Kit (Beyotime, Cat. No. C3606), respectively, according to the manufacturers’ instructions: Adherent PC12 cells were washed with pre-cooled PBS, digested with a trypsin–EDTA solution (Beyotime, Cat. No. C0201), and centrifuged (200×g, RT, 10 min) to collect pellets, which were subsequently washed by centrifugation (600×g, 4 ◦C, 5 min). ..

    Article Title: hUCMSC mitochondrial EVs confer neuroprotection after ischemia by Tom1l2-mediated mitochondrial fusion and Crls1–cardiolipin axis reprogramming
    Article Snippet: .. Mitochondria were isolated from treated PC12 cells and fresh rat brain tissue using the Cell Mitochondrial Isolation Kit (Beyotime, Cat. No. C3601) and Tissue Mitochondrial Isolation Kit (Beyotime, Cat. No. C3606), respectively, according to the manufacturers’ instructions: Adherent PC12 cells were washed with pre-cooled PBS, digested with a trypsin–EDTA solution (Beyotime, Cat. No. C0201), and centrifuged (200× g , RT, 10 min) to collect pellets, which were subsequently washed by centrifugation (600× g , 4 °C, 5 min). ..

    Centrifugation:

    Article Title: hUCMSC mitochondrial EVs confer neuroprotection after ischemia by Tom1l2-mediated mitochondrial fusion and Crls1-cardiolipin axis reprogramming.
    Article Snippet: .. Mitochondria were isolated from treated PC12 cells and fresh rat brain tissue using the Cell Mitochondrial Isolation Kit (Beyotime, Cat. No. C3601) and Tissue Mitochondrial Isolation Kit (Beyotime, Cat. No. C3606), respectively, according to the manufacturers’ instructions: Adherent PC12 cells were washed with pre-cooled PBS, digested with a trypsin–EDTA solution (Beyotime, Cat. No. C0201), and centrifuged (200×g, RT, 10 min) to collect pellets, which were subsequently washed by centrifugation (600×g, 4 ◦C, 5 min). ..

    Article Title: hUCMSC mitochondrial EVs confer neuroprotection after ischemia by Tom1l2-mediated mitochondrial fusion and Crls1–cardiolipin axis reprogramming
    Article Snippet: .. Mitochondria were isolated from treated PC12 cells and fresh rat brain tissue using the Cell Mitochondrial Isolation Kit (Beyotime, Cat. No. C3601) and Tissue Mitochondrial Isolation Kit (Beyotime, Cat. No. C3606), respectively, according to the manufacturers’ instructions: Adherent PC12 cells were washed with pre-cooled PBS, digested with a trypsin–EDTA solution (Beyotime, Cat. No. C0201), and centrifuged (200× g , RT, 10 min) to collect pellets, which were subsequently washed by centrifugation (600× g , 4 °C, 5 min). ..

    Single Cell:

    Article Title: Molybdenum and cadmium co-induced apoptosis and necroptosis via activating endoplasmic reticulum stress and upregulating Caspase-8 expression in duck hepatocytes
    Article Snippet: .. Primary duck hepatocytes were dissociated into single-cell suspensions using Trypsin-EDTA Solution (Beyotime, China). .. Subsequently, the cells were stained with YO-PRO-1/PI (C1075S, Beyotime, China) as per the provided protocol.

    Tissue:

    Article Title: hUCMSC mitochondrial EVs confer neuroprotection after ischemia by Tom1l2-mediated mitochondrial fusion and Crls1–cardiolipin axis reprogramming
    Article Snippet: .. Mitochondria were isolated from treated PC12 cells and fresh rat brain tissue using the Cell Mitochondrial Isolation Kit (Beyotime, Cat. No. C3601) and Tissue Mitochondrial Isolation Kit (Beyotime, Cat. No. C3606), respectively, according to the manufacturers’ instructions: Adherent PC12 cells were washed with pre-cooled PBS, digested with a trypsin–EDTA solution (Beyotime, Cat. No. C0201), and centrifuged (200× g , RT, 10 min) to collect pellets, which were subsequently washed by centrifugation (600× g , 4 °C, 5 min). ..

    CCK-8 Assay:

    Article Title: Injectable Thermosensitive Composite Hydrogels for Sustained Nanoparticle Delivery and Enhanced Wound Healing
    Article Snippet: HaCaT cells and HaCaT cell-specific culture media were purchased from Procell Life Science & Technology Co., Ltd. (Hubei, China). .. Trypsin-EDTA solution (0.25% trypsin), Cell Counting Kit-8 (CCK-8), the Calcein-AM/propidium iodide (PI) live/dead staining kit, and the ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). ..

    Article Title: Injectable Thermosensitive Composite Hydrogels for Sustained Nanoparticle Delivery and Enhanced Wound Healing.
    Article Snippet: HaCaT cells and HaCaT cell-specific culture media were purchased from Procell Life Science & Technology Co., Ltd. (Hubei, China). .. Trypsin-EDTA solution (0.25% trypsin), Cell Counting Kit-8 (CCK-8), the Calcein-AM/propidium iodide (PI) live/dead staining kit, and the ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). ..

    Staining:

    Article Title: Injectable Thermosensitive Composite Hydrogels for Sustained Nanoparticle Delivery and Enhanced Wound Healing
    Article Snippet: HaCaT cells and HaCaT cell-specific culture media were purchased from Procell Life Science & Technology Co., Ltd. (Hubei, China). .. Trypsin-EDTA solution (0.25% trypsin), Cell Counting Kit-8 (CCK-8), the Calcein-AM/propidium iodide (PI) live/dead staining kit, and the ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). ..

    Article Title: Injectable Thermosensitive Composite Hydrogels for Sustained Nanoparticle Delivery and Enhanced Wound Healing.
    Article Snippet: HaCaT cells and HaCaT cell-specific culture media were purchased from Procell Life Science & Technology Co., Ltd. (Hubei, China). .. Trypsin-EDTA solution (0.25% trypsin), Cell Counting Kit-8 (CCK-8), the Calcein-AM/propidium iodide (PI) live/dead staining kit, and the ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). ..

    ROS Assay:

    Article Title: Injectable Thermosensitive Composite Hydrogels for Sustained Nanoparticle Delivery and Enhanced Wound Healing
    Article Snippet: HaCaT cells and HaCaT cell-specific culture media were purchased from Procell Life Science & Technology Co., Ltd. (Hubei, China). .. Trypsin-EDTA solution (0.25% trypsin), Cell Counting Kit-8 (CCK-8), the Calcein-AM/propidium iodide (PI) live/dead staining kit, and the ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). ..

    Article Title: Injectable Thermosensitive Composite Hydrogels for Sustained Nanoparticle Delivery and Enhanced Wound Healing.
    Article Snippet: HaCaT cells and HaCaT cell-specific culture media were purchased from Procell Life Science & Technology Co., Ltd. (Hubei, China). .. Trypsin-EDTA solution (0.25% trypsin), Cell Counting Kit-8 (CCK-8), the Calcein-AM/propidium iodide (PI) live/dead staining kit, and the ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). ..



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